TY - JOUR
T1 - A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing
AU - Kellner, Max J
AU - Ross, James J
AU - Schnabl, Jakob
AU - Dekens, Marcus P S
AU - Matl, Martin
AU - Heinen, Robert
AU - Grishkovskaya, Irina
AU - Bauer, Benedikt
AU - Stadlmann, Johannes
AU - Menéndez-Arias, Luis
AU - Straw, Andrew D
AU - Fritsche-Polanz, Robert
AU - Traugott, Marianna
AU - Seitz, Tamara
AU - Zoufaly, Alexander
AU - Födinger, Manuela
AU - Wenisch, Christoph
AU - Zuber, Johannes
AU - Pauli, Andrea
AU - Brennecke, Julius
N1 - Copyright © 2022 Kellner, Ross, Schnabl, Dekens, Matl, Heinen, Grishkovskaya, Bauer, Stadlmann, Menéndez-Arias, Straw, Fritsche-Polanz, Traugott, Seitz, Zoufaly, Födinger, Wenisch, Zuber, Vienna COVID-19 Detection Initiative (VCDI), Pauli and Brennecke.
PY - 2022
Y1 - 2022
N2 - RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use. Loop-mediated isothermal amplification after reverse transcription (RT-LAMP) is an alternative nucleic acid detection method that overcomes these limitations. Here, we present a rapid, robust, and sensitive RT-LAMP-based SARS-CoV-2 detection assay. Our 40-min procedure bypasses the RNA isolation step, is insensitive to carryover contamination, and uses a colorimetric readout that enables robust SARS-CoV-2 detection from various sample types. Based on this assay, we have increased sensitivity and scalability by adding a nucleic acid enrichment step (Bead-LAMP), developed a version for home testing (HomeDip-LAMP), and identified open-source RT-LAMP enzymes that can be produced in any molecular biology laboratory. On a dedicated website, rtlamp.org (DOI: 10.5281/zenodo.6033689), we provide detailed protocols and videos. Our optimized, general-purpose RT-LAMP assay is an important step toward population-scale SARS-CoV-2 testing.
AB - RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use. Loop-mediated isothermal amplification after reverse transcription (RT-LAMP) is an alternative nucleic acid detection method that overcomes these limitations. Here, we present a rapid, robust, and sensitive RT-LAMP-based SARS-CoV-2 detection assay. Our 40-min procedure bypasses the RNA isolation step, is insensitive to carryover contamination, and uses a colorimetric readout that enables robust SARS-CoV-2 detection from various sample types. Based on this assay, we have increased sensitivity and scalability by adding a nucleic acid enrichment step (Bead-LAMP), developed a version for home testing (HomeDip-LAMP), and identified open-source RT-LAMP enzymes that can be produced in any molecular biology laboratory. On a dedicated website, rtlamp.org (DOI: 10.5281/zenodo.6033689), we provide detailed protocols and videos. Our optimized, general-purpose RT-LAMP assay is an important step toward population-scale SARS-CoV-2 testing.
U2 - 10.3389/fmolb.2022.801309
DO - 10.3389/fmolb.2022.801309
M3 - Article
C2 - 35433827
SN - 2296-889X
VL - 9
SP - 801309
JO - Frontiers in Molecular Biosciences
JF - Frontiers in Molecular Biosciences
ER -